Prevalence of IgM and IgG anti-Toxoplasma gondii antibodies in university students from the Biological Sciences Course, Pontal do Triângulo Mineiro

Article Sidebar

PDF (Português (Brasil)) XML (Português (Brasil)) HTML (Português (Brasil))
Published: Dec 17, 2020
DOI: https://doi.org/10.21876/rcshci.v10i4.1038
Keywords:
students, Toxoplasma gondii infection, antibodies

Main Article Content

Isadora Paula Franco dos Santos
Federal University of Uberlândia, Institute of Exact and Natural Sciences of Pontal, Laboratory of Biomedical Sciences
Ana Flávia Santos Silva Marinho
Federal University of Uberlândia, Institute of Exact and Natural Sciences of Pontal, Laboratory of Biomedical Sciences
César Gómez-Hernández
Federal University of Triângulo Mineiro, Laboratory of Immunology
https://orcid.org/0000-0003-2792-8485
Cristhianne Molinero Ratkevicius Andrade
Federal University of Triângulo Mineiro, Laboratory of Immunology
https://orcid.org/0000-0003-3469-0022
Virmondes Rodrigues
Federal University of Triângulo Mineiro, Laboratory of Immunology
https://orcid.org/0000-0001-8706-4223
Karine Rezende-Oliveira
Federal University of Uberlândia, Institute of Exact and Natural Sciences of Pontal, Laboratory of Biomedical Sciences
https://orcid.org/0000-0002-1220-6052

Abstract

Objective: To investigate the presence of anti-Toxoplasma gondii IgM and IgG in the serum of Biological Sciences students at the Federal University of Uberlândia, Pontal campus. Methods: Cross-sectional prevalence study with data collection was conducted between June 2017 and February 2018. Indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA) techniques were used, and semi-structured questionnaires were used to assess previous knowledge abouttoxoplasmosis. Results: 128 students aged 18 to 41 years participated in the study, 83 (64.4%) female and 45 (35.6%) male. Blood samples were collected from 106 participants, and 13 (12.3%) had anti-T gondii antibodies. Of these, 3 (2.8%) claimed to consume unpasteurized bovine milk, 3 (2.8%) raw or undercooked meat, and 1 (0.94%) raw or unhygienic vegetables. There was no statistically significant association between positivity (IgM anti-T. gondii or IgG anti-T. gondii) and the variables analyzed in the questionnaire. Conclusion: In this work, it was possible to verify the presence of IgM and IgG anti-T gondii antibodies in university students. There was no association between seropositivity and eating habits. However, the questionnaire response analysis showed risky behavior on these individuals, especially concerning frequent contact with dogs and cats, often collected on the street.

Article Details

How to Cite
1.
Franco dos Santos IP, Marinho AFSS, Gómez-Hernández C, Andrade CMR, Rodrigues V, Rezende-Oliveira K. Prevalence of IgM and IgG anti-Toxoplasma gondii antibodies in university students from the Biological Sciences Course, Pontal do Triângulo Mineiro. HSJ [Internet]. 2020 Dec. 17 [cited 2024 Nov. 22];10(4):151-9. Available from: https://portalrcs.hcitajuba.org.br/index.php/rcsfmit_zero/article/view/1038
Issue
Vol. 10 No. 4 (2020): October to December 2020
Section
ORIGINAL ARTICLE

Authors maintain copyright and grant the HSJ the right to first publication. From 2024, the publications wiil be licensed under Attribution 4.0 International , allowing their sharing, recognizing the authorship and initial publication in this journal.

Authors are authorized to assume additional contracts separately for the non-exclusive distribution of the version of the work published in this journal (e.g., publishing in an institutional repository or as a book chapter), with acknowledgment of authorship and initial publication in this journal.

Authors are encouraged to publish and distribute their work online (e.g., in institutional repositories or on their personal page) at any point after the editorial process.

Also, the AUTHOR is informed and consents that the HSJ can incorporate his article into existing or future scientific databases and indexers, under the conditions defined by the latter at all times, which will involve, at least, the possibility that the holders of these databases can perform the following actions on the article.

Author Biographies

Isadora Paula Franco dos Santos, Federal University of Uberlândia, Institute of Exact and Natural Sciences of Pontal, Laboratory of Biomedical Sciences

Graduated in Biological Sciences at the Institute of Exact and Natural Sciences of Pontal, Federal University of Uberlândia. Ituiutaba, Minas Gerais, Brazil.

Ana Flávia Santos Silva Marinho, Federal University of Uberlândia, Institute of Exact and Natural Sciences of Pontal, Laboratory of Biomedical Sciences

Graduated in Biological Sciences at the Institute of Exact and Natural Sciences of Pontal, Federal University of Uberlândia. Ituiutaba, Minas Gerais, Brazil.

César Gómez-Hernández, Federal University of Triângulo Mineiro, Laboratory of Immunology

PhD in Tropical Medicine and Infectious Diseases at the Federal University of Triângulo Mineiro. Uberaba, Minas Gerais, Brazil.

Cristhianne Molinero Ratkevicius Andrade, Federal University of Triângulo Mineiro, Laboratory of Immunology

Master in Health Sciences from the Federal University of Triângulo Mineiro. Uberaba, Minas Gerais, Brazil

Virmondes Rodrigues, Federal University of Triângulo Mineiro, Laboratory of Immunology

PhD in Immunology from the University of São Paulo. Titular Professor of the Immunology Discipline at the Federal University of Triângulo Mineiro. Uberaba, Minas Gerais, Brazil.

Karine Rezende-Oliveira, Federal University of Uberlândia, Institute of Exact and Natural Sciences of Pontal, Laboratory of Biomedical Sciences

PhD in Tropical Medicine and Infectious Diseases, Federal University of Triângulo Mineiro. Post-Doctorate in Tropical Medicine and Infectious Diseases. Associate Professor II of the disciplines of Immunology, Parasitology, Epidemiology and Public Health, Parasitological Methods of Diagnosis and Immunological and Molecular Aspects of the Parasite-Host Relationship in the Biological Sciences course at the Federal University of Uberlândia, Faculty of Integrated Sciences of Pontal (FACIP). Ituiutaba, Minas Gerais, Brazil.

References

Dubey JP. Toxoplasmosis - a waterborne zoonosis. Veterinary parasitology. 2004;126(1-2):57-72. https://doi.org/10.1016/j.vetpar.2004.09.005 PMid:15567579

Zajdenweber M, Muccioli C, Belfort R Jr. Acometimento ocular em pacientes com AIDS e toxoplasmose do sistema nervoso central: antes e depois do HAART. Arq Bras Oftalmol. 2005;68(6):773-5. https://doi.org/10.1590/s0004-27492005000600012 PMID: 17344978

Luft BJ, Remington JS. Toxoplasmic encephalitis in AIDS. Clin Infect Dis. 1992;15(2):211-22. https://doi.org/10.1093/clinids/15.2.211 PMID:1520757.

Robert-Gangneux F, Dardé ML. Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev. 2012;25(2):264-96. https://doi.org/10.1128/cmr.05013-11. Erratum in: Clin Microbiol Rev. 2012;25(3):583. PMID:22491772 PMCID:PMC3346298

Yamamoto YI, Mourad AM, Prescendo FR, Ribeiro KS, Oliveira CB, Garcia RSL. Seroepidemiological survey of Toxoplasma gondii infection among students of a university. Rev Bras An Clin [Internet]. 2009 [cited 2020 Nov 24];41(4):299-302. Avaiable from: http://www.rbac.org.br/wp-content/uploads/2016/08/RBAC_Vol41_n4-Completa.pdf

Maia LP, Oliveira KR, Ferreira GLS, Gómez-Hernández C, Nomeline QSS, Aidar FLM. The prevalence of toxoplasmosis in the Pontal of the Triângulo Mineiro region, Minas Gerais State, Brazil. Rev Patol Trop. 2012;41(4):457-64. https://doi.org/10.5216/rpt.v41i4.21707

Guimarães AM, Ribeiro MFB, Lima JD, Cury MC, Spiewak G. Freqüência de anticorpos anti-Toxoplasma gondii em cães de Belo Horizonte, Minas Gerais. Arq Bras Med Vet Zootec. 1992;44(67-68).

Rodrigues JP, Frei F, Navarro IT, Silva LP, Marcelino MY, de Andrade-Junior HF, de Faria CA, Santos M, Ribeiro-Paes JT. Seroepidemiological analysis of toxoplasmosis in college students. J Venom Anim Toxins Incl Trop Dis. 2015;21(1):1. https://doi.org/10.1186/1678-9199-21-1 PMID:25587248 PMCID:PMC4293106.

Obaid HM. Survey study on toxoplasmosis among Kirkuk university students. J Univer Kerbala [Internet]. 2017 [cited 2020 Nov 24];13(1):253-9. Avaiable from: https://kj.uokerbala.edu.iq/article_123452.html

Passos ADC, Bollela VR, Furtado JMF, Lucena MM, Bellissimo-Rodrigues F, Paula JS, et al. Prevalence and risk factors of toxoplasmosis among adults in a small Brazilian city. Rev Soc Bras Med Trop. 2018;51(6):781-7. https://doi.org/10.1590/0037-8682-0214-2017 PMID: 30517531

Egorov AI, Converse R, Griffin SM, Styles J, Klein E, Sams E, et al. Environmental risk factors for Toxoplasma gondii infections and the impact of latent infections on allostatic load in residents of Central North Carolina. BMC Infec Dis. 2018;18(1):421. https://doi.org/10.1186/s12879-018-3343-y PMid:30139351 PMCid:PMC6108134

Garcia JL, Navarro IT, Ogawa L, Oliveira RC. Seroprevalence of Toxoplasma gondii in swine, bovine, ovine and equine, and their correlation with human, felines and canines, from farms in north region of Paraná state, Brazil. Cienc Rural. 1999;29(1):91-7. https://doi.org/10.1590/S0103-84781999000100017

Amendoeira MRR, Camillo-Coura LF. Brief review on toxoplasmosis in pregnancy. Sci Med [Internet]. 2010 [cited 2020 Nov 24];20(1):113-9. Avaiable from: https://revistaseletronicas.pucrs.br/ojs/index.php/scientiamedica/article/view/5917/4953

Leão PRD, Meirelles-Filho J, Medeiros SF. Toxoplasmosis: seroprevalence in postpartum women attended by SUS (Brazilian Public Health System). Rev Bras Ginecol Obstet. 2004;26(8):627-32. https://doi.org/10.1590/S0100-72032004000800006

Omata Y, Igarashi M, Ramos MI, Nakabayashi T. Toxoplasma gondii: antigenic differences between endozoites and cystozoites defined by monoclonal antibodies. Parasitol Res. 1989;75(3):189-93. https://doi.org/10.1007/bf00931274 PMID:2469081