Antibacterial and antifungal activity of curcumin and methylene blue associated with laser on bacterial and fungal strains

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Published: Dec 28, 2023
DOI: https://doi.org/10.21876/rcshci.v13i4.1454
Keywords:
Bacterial infections, Curcumin, Fungal Infections, In vitro technique, Microbial drug resistance, Photochemotherapy, Photosensitizer

Main Article Content

Brenda Ellen Meneses Cardoso
Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba
Ana Andreza Albuquerque Medeiros
Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba
Matheus Lopes Martins
Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba
André Luca Araujo de Sousa
State University of Piauí
Nathanael dos Santos Alves
Parnaíba Delta Federal University
Leiz Maria Costa Veras
Parnaíba Delta Federal University
Alyne Rodrigues Araújo
Parnaíba Delta Federal University
Antonione Santos Bezerra Pinto
Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba

Abstract

Objective: To analyze the effect of methylene blue and 10% curcumin in fungi and bacteria through an in vitro study using photodynamic therapy (PDT). Methods: Curcumin and methylene blue were photosensitized by a Photon Lase III laser applied for 90 s in a dark environment within a laminar flow chamber. Enterococcus faecalis and Candida albicans strains were cultured and standardized. Then, a minimum inhibitory concentration (MIC) assay was conducted for these photosensitizers, with concentration variations and incubation to evaluate their antimicrobial activity. Results: With PDT, Curcumin had significant antibacterial activity against E. faecalis (MIC = 250 µg/mL). In contrast, methylene blue had antibacterial activity against E. faecalis (MIC < 12.5 µg/mL with PDT) and antifungal activity against C. albicans (MIC <12.5 µg/mL with or without PDT). Both agents showed greater efficacy in the presence of the laser. The results suggest that curcumin and methylene blue associated with laser may effectively treat microbial infections. Conclusion: Further research is needed to evaluate the efficacy and safety of using these agents in animal and human models and their effectiveness against different bacterial and fungal strains.


 

Article Details

How to Cite
1.
Meneses Cardoso BE, Albuquerque Medeiros AA, Lopes Martins M, Araujo de Sousa AL, dos Santos Alves N, Costa Veras LM, Araújo AR, Pinto ASB. Antibacterial and antifungal activity of curcumin and methylene blue associated with laser on bacterial and fungal strains. HSJ [Internet]. 2023Dec.28 [cited 2024May20];13(4):33-7. Available from: https://portalrcs.hcitajuba.org.br/index.php/rcsfmit_zero/article/view/1454
Issue
Vol 13 No 4 (2023): October to December 2023
Section
ORIGINAL ARTICLE
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Author Biographies

Brenda Ellen Meneses Cardoso, Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba

Undergraduate Medical Student at Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba.

Ana Andreza Albuquerque Medeiros, Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba

Undergraduate Medical Student at Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba.

Matheus Lopes Martins, Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba

Undergraduate Medical Student at Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba.

André Luca Araujo de Sousa, State University of Piauí

Dentistry Student at the State University of Piauí.

Nathanael dos Santos Alves, Parnaíba Delta Federal University

Biodiversiy and Biotecnology Research Center (BIOTEC), Parnaíba Delta Federal University.

Leiz Maria Costa Veras, Parnaíba Delta Federal University

Medical Professor at the Federal University of Piauí.

Alyne Rodrigues Araújo, Parnaíba Delta Federal University

Medical Professor at the Parnaíba Delta Federal University.

Antonione Santos Bezerra Pinto, Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba

PhD in Morphofunctional Sciences and Medical Professor at Faculty of Human, Exact and Health Sciences of Piauí of the Higher Education Institute of the Vale do Parnaíba.

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