Antibacterial and antifungal activity of curcumin and methylene blue associated with laser on bacterial and fungal strains
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Resumo
Objective: To analyze the effect of methylene blue and 10% curcumin in fungi and bacteria through an in vitro study using photodynamic therapy (PDT). Methods: Curcumin and methylene blue were photosensitized by a Photon Lase III laser applied for 90 s in a dark environment within a laminar flow chamber. Enterococcus faecalis and Candida albicans strains were cultured and standardized. Then, a minimum inhibitory concentration (MIC) assay was conducted for these photosensitizers, with concentration variations and incubation to evaluate their antimicrobial activity. Results: With PDT, Curcumin had significant antibacterial activity against E. faecalis (MIC = 250 µg/mL). In contrast, methylene blue had antibacterial activity against E. faecalis (MIC < 12.5 µg/mL with PDT) and antifungal activity against C. albicans (MIC <12.5 µg/mL with or without PDT). Both agents showed greater efficacy in the presence of the laser. The results suggest that curcumin and methylene blue associated with laser may effectively treat microbial infections. Conclusion: Further research is needed to evaluate the efficacy and safety of using these agents in animal and human models and their effectiveness against different bacterial and fungal strains.
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Referências
Bourbour S, Darbandi A, Bostanghadiri N, Ghanavati R, Taheri B, Bahador A. Effects of Antimicrobial photosensitizers of Photodynamic Therapy (PDT) to Treat Periodontitis. Curr Pharm Biotechnol. 2023 Jul 20. Epub ahead of print. PMID: 37475551 https://doi.org/10.2174/1389201024666230720104516 DOI: https://doi.org/10.2174/1389201024666230720104516
Yuan Y, Liu Q, Huang Y, Qi M, Yan H, Li W, Zhuang H. Antibacterial Efficacy and Mechanisms of Curcumin-Based Photodynamic Treatment against Staphylococcus aureus and Its Application in Juices. Molecules. 2022;27(20):7136. https://doi.org/10.3390/molecules27207136 PMID: 36296729 PMCID: PMC9612228 DOI: https://doi.org/10.3390/molecules27207136
La Selva A, Negreiros RM, Bezerra DT, Rosa EP, Pavesi VCS, Navarro RS, et al. Treatment of herpes labialis by photodynamic therapy. Medicine. 2020;99(12):e19500. https://doi.org/10.1097/MD.0000000000019500 PMid:32195950 PMCid:PMC7220348 DOI: https://doi.org/10.1097/MD.0000000000019500
Santezi C, Reina BD, Dovigo LN. Curcumin-mediated Photodynamic Therapy for the treatment of oral infections-A review. Photodiagnosis Photodyn Ther. 2018;21:409-15. https://doi.org/10.1016/j.pdpdt.2018.01.016 PMID: 29378256 DOI: https://doi.org/10.1016/j.pdpdt.2018.01.016
Gugulothu D, Desai P, Patravale V. A versatile liquid chromatographic technique for pharmacokinetic estimation of curcumin in human plasma. J Chromatogr Sci. 2014;52(8):872-9. https://doi.org/10.1093/chromsci/bmt131 PMID: 24036005 DOI: https://doi.org/10.1093/chromsci/bmt131
Xie L, Ji X, Zhang Q, Wei Y. Curcumin combined with photodynamic therapy, promising therapies for the treatment of cancer. Biomed Pharmacother. 2022;146:112567. https://doi.org/10.1016/j.biopha.2021.112567 PMid:34953392 DOI: https://doi.org/10.1016/j.biopha.2021.112567
Ailioaie LM, Litscher G. Curcumin and Photobiomodulation in Chronic Viral Hepatitis and Hepatocellular Carcinoma. Int J Mol Sci. 2020 ;21(19):7150. https://doi.org/10.3390/ijms21197150 PMid:32998270 PMCid:PMC7582680 DOI: https://doi.org/10.3390/ijms21197150
Entezari S, Moezzimoghadam N, Lawaf S, Azizi A. In vitro Effect of Photodynamic Therapy with Curcumin and Methylene Blue Photosensitizers on Staphylococcus Aureus. J Dent (Shiraz, Iran). 2022;23(2 Suppl):387-92. https://doi.org/10.30476/dentjods.2021.90146.1470
Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing. 25th ed. CLSI supplement M100. Wayne, PA: CLSI; 2015.
Clinical and Laboratory Standards Institute (CLSI). Reference method for broth dilution antifungal susceptibility testing of yeasts. 3rd ed. CLSI standard M27. Wayne, PA: CLSI; 2008.
Kiamahalleh MV, Najafpour-Darzi G, Rahimnejad M, Moghadamnia AA, Valizadeh Kiamahalleh M. High performance curcumin subcritical water extraction from turmeric (Curcuma longa L.). J Chromatogr B Analyt Technol Biomed Life Sci. 2016;1022:191-8. https://doi.org/10.1016/j.jchromb.2016.04.021 PMID: 27107245 DOI: https://doi.org/10.1016/j.jchromb.2016.04.021
Herculano LS, Kalschne DL, Canan C, Reis TS, Marcon CT, Benetti VP, Malacarne LC, Blanco K, Bagnato VS. Antimicrobial curcumin-mediated photodynamic inactivation of bacteria in natural bovine casing. Photodiagnosis Photodyn Ther. 2022;40:103173. https://doi.org/10.1016/j.pdpdt.2022.103173 PMID: 36307061 DOI: https://doi.org/10.1016/j.pdpdt.2022.103173
Trigo-Gutierrez JK, Vega-Chacón Y, Soares AB, Mima EGO. Antimicrobial Activity of Curcumin in Nanoformulations: A Comprehensive Review. Int J Mol Sci. 2021;22(13):7130. https://doi.org/10.3390/ijms22137130 PMID: 34281181 PMCID: PMC8267827 DOI: https://doi.org/10.3390/ijms22137130
Concilio S, Sessa L, Petrone AM, Porta A, Diana R, Iannelli P, Piotto S. Structure Modification of an Active Azo-Compound as a Route to New Antimicrobial Compounds. Molecules. 2017;22(6):875. https://doi.org/10.3390/molecules22060875 PMID: 28587076 PMCID: PMC6152751 DOI: https://doi.org/10.3390/molecules22060875
Prathyusha E, A P, Ahmed H, Dethe MR, Agrawal M, Gangipangi V, Sudhagar S, Krishna KV, Dubey SK, Pemmaraju DB, Alexander A. Investigation of ROS generating capacity of curcumin-loaded liposomes and its in vitro cytotoxicity on MCF-7 cell lines using photodynamic therapy. Photodiagnosis Photodyn Ther. 2022;40:103091. https://doi.org/10.1016/j.pdpdt.2022.103091 PMid:36031144 DOI: https://doi.org/10.1016/j.pdpdt.2022.103091
Varzandeh M, Mohammadinejad R, Esmaeilzadeh-Salestani K, Dehshahri A, Zarrabi A, Aghaei-Afshar A. Photodynamic therapy for leishmaniasis: Recent advances and future trends. Photodiagnosis Photodyn Ther. 2021;36:102609. https://doi.org/10.1016/j.pdpdt.2021.102609 PMid:34728420 DOI: https://doi.org/10.1016/j.pdpdt.2021.102609
Afrasiabi S, Partoazar A, Chiniforush N, Goudarzi R. The Potential Application of Natural Photosensitizers Used in Antimicrobial Photodynamic Therapy against Oral Infections. Pharmaceuticals. 2022;15(6):767. https://doi.org/10.3390/ph15060767 PMid:35745686 PMCid:PMC9227410 DOI: https://doi.org/10.3390/ph15060767
Dias LD, Blanco KC, Mfouo-Tynga IS, Inada NM, Bagnato VS. Curcumin as a photosensitizer: From molecular structure to recent advances in antimicrobial photodynamic therapy. J Photochem Photobiol C: Photochem Rev. 2020;45:100384.
https://doi.org/10.1016/j.jphotochemrev.2020.100384 DOI: https://doi.org/10.1016/j.jphotochemrev.2020.100384
Wu J, Mou H, Xue C, Leung AW, Xu C, Tang QJ. Photodynamic effect of curcumin on Vibrio parahaemolyticus. Photodiagnosis Photodyn Ther. 2016;15:34-9. https://doi.org/10.1016/j.pdpdt.2016.05.004 PMid:27177810 DOI: https://doi.org/10.1016/j.pdpdt.2016.05.004