Desenvolvimento de um Modelo Experimental de Dislipidemia de Baixo Custo/Development of low Cost Dislipidemia Experimental Model
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Abstract
Objetivo: Desenvolver um novo método experimental de baixo custo para indução de dislipidemia em ratos. Materiais e Métodos: Foram utilizados 20 ratos, da linhagem Wistar, divididos em dois grupos (n=10). O grupo 1 (controle) recebeu ração padrão para ratos da marca Purina® (com concentração padrão de colesterol) e o grupo 2 recebeu a mesma ração adicionada de 0,5% p/p de colesterol, obtido através da gema de ovo (grupo experimental), por 50 dias. Resultados: o grupo tratado com ração padrão da marca Purina® adicionada de 0,5% p/p de colesterolapresentou aumento significativo dos níveis séricos de colesterol total (118,6± 4,74 vs. 84,2±5,0 mg/dL, p<0,01 ), LDL-C (54,4± 7,9 vs. 23,6 ±7,0 mg/dL, p<0,01), VLDL-C (45,9±1,2 vs. 29,0± 5,8 mg/dL, p<0,01) e triglicérides (229,3±6,0 vs 145,0± 28,9 mg/dL, p<0,01) e redução significativa do HDL-C (18,3±4,8 vs. 31,7±5,6 mg/dL, p<0,01), quando comparado ao grupo controle. Conclusão: A utilização da ração padrão da marca Purina® adicionada de 0,5% p/p de colesterol mostrou-se eficaz em produzir alterações significativas nos níveis séricos de colesterol total, LDL-colesterol, VLDL-colesterol, HDL-colesterol e triglicérides, demonstrando que este modelo experimental de baixo custo constitui uma ferramenta útil para produzir dislipidemia em ratos.
Palavras-Chave: Dislipidemia, modelo experimental, ratos.
ABSTRACT
Objective: Development of a new experimental low-cost method for induction of dislipidemia in rats. Materials and Methods: Twenty male Wistar rats were used, allocated in two groups (n = 10). Group 1 (control) received a standard diet for rats Purina® mark (with a standard concentration of cholesterol) and group 2 received the same chow and 0.5% p/p cholesterol obtained through yolk (Group experimental) for 50 days. Results: The group treated with the standard ration Purina® branded and 0.5% p/p cholesterol showed a significant increase in serum total cholesterol (118,6± 4,74 vs. 84,2±5,0 mg/dL, p<0,01 ), LDL-C (54,4± 7,9 vs. 23,6 ±7,0mg/dL, p<0,01), VLDL-C (45,9±1,2 vs. 29,0± 5,8 mg/dL, p<0,01) and triglycerides (229,3±6,0 vs 145,0± 28,9 mg/dL, p<0,01) and significant reduction of HDL-C (18,3±4,8 vs. 31,7±5,6 mg/dL, p<0,01) when compared to the control group. Conclusion: The use of standard ration Purina® branded and added to 0.5% p/p cholesterol was effective in producing significant changes in serum levels of total cholesterol, LDL-cholesterol, VLDL-cholesterol, HDL-cholesterol and triglycerides, demonstrating that this experimental model makes a useful low cost tool to produce dyslipidemia in rats.
Keywords: Dyslipidemia, experimental model, rats
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