Adult human neural cells in culture following traumatic brain injury

Main Article Content

Marco Aurelio M Freire
https://orcid.org/0000-0002-6483-0498
Starlynn Freire dos Santos
Gabriel Sousa Rocha
https://orcid.org/0000-0001-7101-8492
Ianara Mendonça Costa
Lucidio Clebeson Oliveira
https://orcid.org/0000-0002-2033-7546
Fausto Pierdoná Guzen
Daniel Falcão
José Rodolfo Lopes P Cavalcanti
https://orcid.org/0000-0002-1554-3249

Abstract

Objective: The present study aims to evaluate the viability of adult human neural cells in culture obtained from traumatized brain tissues collected in emergency surgery procedures. Methods: Exploratory, descriptive, quantitative and cross-sectional study evaluating samples obtained from patients who underwent traumatic brain injury with extrusion of brain tissue submitted to cell culture in a standardized medium, being preserved during 168h. After observation under phase contrast microscopy and immunohistochemical processing for neuronal (MAP-2) and glial (GFAP) markers, morphometric parameters of neural cells (cell body area, dendritic field length and fractal dimension) were evaluated using ImageJ software, with data obtained after 24, 72 and 168h being compared using non-parametric Kruskal Wallis test, followed by Dunn’s post hoc test. Results: The explant of the nervous tissue revealed a consolidated pattern of cell migration into the culture medium. Cell proliferation, upon reaching confluence, presented an aspect of cellular distribution juxtaposed along the culture medium at all time points analyzed. Both neurons and glial cells remained viable after 168h in culture, with their morphologies not varying significantly throughout the time points evaluated. Immunohistochemistry for MAP-2 showed a relatively well-preserved cytoskeletal organization. GFAP immunoreactivity revealed activated astrocytes especially at the later time point. Conclusions: Our results point out the viability of cell culture from traumatized human nervous tissue, opening up perspectives for the use of substances of natural origin that may contribute neuroprotectively to neuronal maintenance in culture, allowing future translational approach.



Article Details

How to Cite
1.
Freire MAM, Santos SF dos, Rocha GS, Costa IM, Oliveira LC, Guzen FP, et al. Adult human neural cells in culture following traumatic brain injury. HSJ [Internet]. 2023 Sep. 17 [cited 2024 Nov. 13];13(3):23-30. Available from: https://portalrcs.hcitajuba.org.br/index.php/rcsfmit_zero/article/view/1422
Section
ORIGINAL ARTICLE
Author Biographies

Marco Aurelio M Freire, State University of Rio Grande do Norte

Biologist, PhD in Neurosciences and Cell Biology from the Federal University of Pará.

Starlynn Freire dos Santos, State University of Rio Grande do Norte

Physician, Master in Health and Society from the State University of Rio Grande do Norte.

Gabriel Sousa Rocha, State University of Rio Grande do Norte

Physical Educator, Master in Biochemistry and Molecular Biology from the State University of Rio Grande do Norte.

Ianara Mendonça Costa, State University of Rio Grande do Norte

Nutritionist, Master in Health and Society from the State University of Rio Grande do Norte.

Lucidio Clebeson Oliveira, State University of Rio Grande do Norte

PhD in Psychobiology from the Federal University of Rio Grande do Norte.

Fausto Pierdoná Guzen, State University of Rio Grande do Norte

PhD in Psychobiology from the Federal University of Rio Grande do Norte.

Daniel Falcão, Virginia Commonwealth University

MD, Specialist in Vascular Neurology at the University of South Florida.

José Rodolfo Lopes P Cavalcanti, State University of Rio Grande do Norte

PhD in Psychobiology from the Federal University of Rio Grande do Norte.

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